FYCO1 Antibody MBS9600315 from MyBioSource.com

Published Figures
Supplier Images

Register or Sign In to View Figures.

Cells
August 06 2021

Fig 1: (A) Endoplasmic reticulum (ER)-localised protrudin forms contact sites with late endosomes (LEs) after detection of Rab7 and phosphoinositide 3 phosphate PI(3)P (yellow) and stopping the movement of LE (STOP). Kinesin-1 bound to protrudin is then handed over to the LE protein FYCO1, which mediates plus-end (+)-directed LE movement (MOVE) along microtubules (MT) to the periphery. Synaptotagmin 7 (SYT7)-mediated LE fusion with the plasma membrane then enables protrusion formation and neurite outgrowth, as previously proposed [14,57]. (B) Overexpression of RTN3 allows the handover of kinesin-1 to occur more rapidly and hence greater numbers of LEs available for protrusion (neurites/axons) at the plasma membrane.
Register or Sign In to View Figures.

Cells
August 06 2021

Fig 2: Knockdown of Protrudin and its interactors attenuates RTN3-induced DRGN neurite outgrowth. (A) Timeline for experimental analysis of Protrudin and its interactors in RTN3-overexpressed DRGN. Representative Western blots to confirm knockdown of (B) Protrudin, (C) FYCO1, (D) RAB7 and (E) SYT7. ß-actin is used as a protein loading control. Representative images to show neurite outgrowth in (F) RTN3 overexpressed DRGN controls and after knockdown of (G) Protrudin, (H) FYCO1, (I) RAB7 and (J) SYT7 in RTN3-overexpressed cultures. (K) Quantification to show attenuation of RTN3-overexpressed DRGN neurite outgrowth by shProtrudin, shFYCO1, shRAB7 and shSYT7. (L) DRGN survival remains unaffected by knockdown of Protrudin, FYCO1, RAB7 and SYT7 in RTN3-overexpressed DRGN. n = 9 wells/condition Scale bars in (F–J) = 100 µm. Data are means ± SEM. *** p = 0.0001, one-way ANOVA with Dunnett’s post hoc test.

Be the first to write a review!

Citations:

(1)

Figures:

Supplier Page

Supplier Page from
MyBioSource.com for
FYCO1 Antibody

Get Pricing

MyBioSource.com's FYCO1 Antibody is a Rabbit Polyclonal antibody. This antibody has been shown to work in applications such as: ELISA, and Western Blot. The FYCO1 Antibody was generated using FYCO1 as the antigen and it reacts with Human, and Rat.

Description

Description: FYCO1 3 isoforms of the human protein are produced by alternative splicing.
Function: May mediate microtubule plus end-directed vesicle transport.
Subunit Structure: Can form homodimers. Interacts (via C-terminus) with MAP1LC3B. Interacts with RAB7A; the interaction with RAB7A induces FYCO1 recruitment to late endosomal/lysosomal compartments. Interacts with MAP1LC3B (By similarity)

Cells

Cells

FYCO1 Antibody from MyBioSource.com

Description

MyBioSource.com

Citations (1)

Reviews

FYCO1 Antibody from MyBioSource.com

About Biocompare

Advertise with Us

Specialized Search Tools

Site Map